Research note: Ukrainian Refugee Crisis Tests Luxembourg’s Humanitarian and Integration Policy

Russia’s invasion of Ukraine and the resulting sanctions have contributed to accelerating the rise in commodity prices (including energy), triggering inflation, and exacerbating instability in an already convalescent Europe. The war has also sparked a massive refugee crisis, the most impressive in speed and size since World War II, whose economic impact is likely to be significant. As of mid-May, the stock of Ukrainian refugees who have fled their country due to the Russian invasion slightly exceeded 6 million, and some 8 million more were displaced internally.1 This refugee crisis imposes huge stress on the humanitarian protection regime and reception capacity of most European countries. This is not only the case in frontline countries such as Poland (3.3 million), Romania (0.9 million), Hungary (0.6 million) or Moldova (0.5 million), but also in other EU member states where the numbers of arrivals has almost reached (and sometimes exceeds) the levels of the 2015-16 refugee crisis in two months only. As of early May, the stocks have risen to 610 thousand in Germany, 70 thousand in France, 40 thousand in Belgium and 5 thousand in Luxembourg. These numbers are likely to grow in the coming weeks, as the war is far from over

A novel dual ionization modality source for infrared laser ablation post-ionization mass spectrometry imaging to study fungicide metabolism and transport

We present a novel probe design for ambient laser-based mass spectrometry imaging combining electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) in a single probe, compatible with a commercial laser ablation electrospray ionization (LAESI) instrument. Here we describe the probe design considerations and features, as well as an in-house developed data processing routine designed to extract accurate mass spectrometry imaging data from ambient laser ablation post-ionization experiments. We characterize the probe performance in both APCI and ESI mode on a selection of compounds and show improved pixel-to-pixel repeatability for LA-APCI as compared to LAESI. We apply the dual ionization probe in APCI mode in a time series experiment to monitor agrochemicals on tomato plants. We investigate the translocation of fungicide isotianil and one of its metabolites, anthranilonitrile, by mass spectrometry imaging over a period of two weeks after application on a leaf surface. LA-APCI-MSI shows translocation of anthranilonitrile from treated leaves towards non-treated leaves. In summary, we demonstrate that LA-APCI imaging is a valuable addition to the ambient mass spectrometry toolbox, with particular advantages for imaging experiments across a variety of compounds

Identification and validation of potential new biomarkers for prostate cancer diagnosis and prognosis using 2D-DIGE and MS

This study was designed to identify and validate potential new biomarkers for prostate cancer and to distinguish patients with and without biochemical relapse. Prostate tissue samples analyzed by 2D-DIGE (two-dimensional difference in gel electrophoresis) and mass spectrometry (MS) revealed downregulation of secernin-1 (P < 0.044) in prostate cancer, while vinculin showed significant upregulation (P < 0.001). Secernin-1 overexpression in prostate tissue was validated using Western blot and immunohistochemistry while vinculin expression was validated using immunohistochemistry. These findings indicate that secernin-1 and vinculin are potential new tissue biomarkers for prostate cancer diagnosis and prognosis, respectively. For validation, protein levels in urine were also examined by Western blot analysis. Urinary vinculin levels in prostate cancer patients were significantly higher than in urine from nontumor patients (P = 0.006). Using multiple reaction monitoring-MS (MRM-MS) analysis, prostatic acid phosphatase (PAP) showed significant higher levels in the urine of prostate cancer patients compared to controls (P = 0.012), while galectin-3 showed significant lower levels in the urine of prostate cancer patients with biochemical relapse, compared to those without relapse (P = 0.017). Three proteins were successfully differentiated between patients with and without prostate cancer and patients with and without relapse by using MRM. Thus, this technique shows promise for implementation as a noninvasive clinical diagnostic technique

Identifikation frühdiagnostischer Tumormarker des rezidivierenden Prostatakarzinoms mittels innovativer Proteomanalysen

Das Prostatakarzinom ist der häufigste maligne Tumor von Männern. Bisher sind jedoch keine Biomarker in der Klinikroutine etabliert, die eine Vorhersage über die Progression eines Prostatakarzinoms ermöglichen. Mit dem Ziel prognostische Biomarker für das rezidivierende Prostatakarzinom zu identifizieren, wurde eine labelfreie Proteomanalyse durchgeführt und eine Methode zur bildgebenden Massenspektrometrie (MSI) entwickelt. Die identifizierten potentiellen Biomarker Mitochondrial Amidoxime Reducing Component 1 (MARC1) und Mitofusin-1 (MNF1) wurden mittels Immunhistochemie verifiziert. Die Ergebnisse der Verifizierung von MARC1 zeigen Potential zur Prognose des Prostatakarzinoms. Im Rahmen der vorliegenden Arbeit wurde zudem eine MSI-Methode zur Analyse von Peptiden aus Formalin-fixierten und Paraffin-eingebetteten Geweben etabliert. Mittels dieser Methode wurde ein Klassifizierungsverfahren zur Differenzierung von rezidivierenden und nicht rezidivierenden Prostatakarzinomen etabliert

A novel dual ionization modality source for infrared laser ablation post-ionization mass spectrometry imaging to study fungicide metabolism and transport

We present a novel probe design for ambient laser-based mass spectrometry imaging combining electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) in a single probe, compatible with a commercial laser ablation electrospray ionization (LAESI) instrument. Here we describe the probe design considerations and features, as well as an in-house developed data processing routine designed to extract accurate mass spectrometry imaging data from ambient laser ablation post-ionization experiments. We characterize the probe performance in both APCI and ESI mode on a selection of compounds and show improved pixel-to-pixel repeatability for LA-APCI as compared to LAESI. We apply the dual ionization probe in APCI mode in a time series experiment to monitor agrochemicals on tomato plants. We investigate the translocation of fungicide isotianil and one of its metabolites, anthranilonitrile, by mass spectrometry imaging over a period of two weeks after application on a leaf surface. LA-APCI-MSI shows translocation of anthranilonitrile from treated leaves towards non-treated leaves. In summary, we demonstrate that LA-APCI imaging is a valuable addition to the ambient mass spectrometry toolbox, with particular advantages for imaging experiments across a variety of compounds

Identification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MS

This study was designed to identify and validate potential new biomarkers for prostate cancer and to distinguish patients with and without biochemical relapse. Prostate tissue samples analyzed by 2D-DIGE (two-dimensional difference in gel electrophoresis) and mass spectrometry (MS) revealed downregulation of secernin-1 (P < 0.044) in prostate cancer, while vinculin showed significant upregulation (P < 0.001). Secernin-1 overexpression in prostate tissue was validated using Western blot and immunohistochemistry while vinculin expression was validated using immunohistochemistry. These findings indicate that secernin-1 and vinculin are potential new tissue biomarkers for prostate cancer diagnosis and prognosis, respectively. For validation, protein levels in urine were also examined by Western blot analysis. Urinary vinculin levels in prostate cancer patients were significantly higher than in urine from nontumor patients (P = 0.006). Using multiple reaction monitoring-MS (MRM-MS) analysis, prostatic acid phosphatase (PAP) showed significant higher levels in the urine of prostate cancer patients compared to controls (P = 0.012), while galectin-3 showed significant lower levels in the urine of prostate cancer patients with biochemical relapse, compared to those without relapse (P = 0.017). Three proteins were successfully differentiated between patients with and without prostate cancer and patients with and without relapse by using MRM. Thus, this technique shows promise for implementation as a noninvasive clinical diagnostic technique